W1: Quality Control in Molecular Diagnostics
(1 day, theoretical)

DATE: 26.5.2021

TARGET AUDIENCE: Beginners to Advanced qPCR users

ENTRANCE QUALIFICATIONS: TATAA Hands-on qPCR module or similar knowledge

DESCRIPTION: This course will give you the tools in how to do proper quality control of your qPCR assays to be used in molecular diagnostics. It will describe which controls are needed and how to the evaluation of experimental data should be performed. The course highlights aspects to be considered with regards to sampling, storage and extraction of nucleic acids. Furthermore, it presents a workflow for qPCR assay validation and how limit of quantification (LOQ) and detection (LOD) are determined.

W2: Sample Preparation and Quality Control of Nucleic Acids
(2 days, with practical part)

DATE: 25.-26.5.2021

TARGET AUDIENCE: Beginners to Medium experienced qPCR users

ENTRANCE QUALIFICATIONS: Basic Molecular biology or similar

DESCRIPTION: One of the most important requirements to get good results from qPCR experiments is to have a template of good quality. In most cases this means having an efficient sample preparation. This course module is focused on extraction of RNA and DNA to be used as template in qPCR and reverse transcription reactions. It also includes the important quality control steps needed to assure reliable results. The course has a practical focus and includes several hands-on exercises.

The course covers topics such as:

  • Overview of nucleic acid extraction methods
  • How to properly determine the concentration of purified nucleic acids
  • Storage of biological samples and purified nucleic acids
  • Quality control of purified material
  • Integrity of purified RNA
  • How to test for the presence of inhibitors

W3: MicroRNA Analysis
(2 days, with practical part)

Learn how to extract and analyze microRNA with appropriate technique!

DATE: 25.-26.5.2021
TARGET AUDIENCE: Researchers interested in analyzing microRNA
ENTRANCE QUALIFICATIONS: Basic knowledge about microRNA and molecular analyses

DESCRIPTION: This course introduces microRNA including its variant forms. Methods to extract and purify microRNA and prepare it for analysis including quality control. Methods for microRNA analysis are presented and compared, to learn how to choose the best method for each study. Analysis of microRNA data is also discussed including challenging normalization.

In the course you will learn:

  • MicroRNA background
  • MiRNA sampling, extraction and quality control
  • Analysis of liquid biopsies
  • MicroRNA analysis with microarray, NGS, RT-qPCR methods
  • Introduction into data analysis